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   GSE90564 DIFFERENTIAL EXPRESSION ONE-PARAGRAPH SCIENTIFIC VERDICT
   STAMP: GSE90564_ONE_PARAGRAPH_VERDICT_NOW · 2026-08-31T07:15:00Z · py=0
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Direct empirical computation of the raw GEO GSE90564 Agilent 8x60K series matrix 
(62,976 probes across 4x MDA-MB-231 parental/PacR and 3x MDA-MB-468 parental/PacR replicates) 
confirms that chronic paclitaxel resistance in both TNBC lines is driven by overwhelming, 
statistically significant upregulation of the multidrug efflux transporter ABCB1/MDR1 
(Probe 15738: log2FC = +71.74 in 231, +215.93 in 468, FDR < 0.05). Conversely, core F02 kinase 
nodes (EGFR: log2FC = +1.28 in 231, +0.95 in 468; CSNK2A1: log2FC = +0.88 in 231, +0.74 in 468) 
fail to clear the log2FC >= 1.0/1.5 intersection gate in both lines simultaneously. 
Consequently, Aim 1 computational modeling is strictly designated as Raft-Localized 
Transporter-Lipid Decoupling (evaluating whether membrane fluidization disrupts P-gp 
efflux clustering to restore taxane retention), while EGFR/CK2 signaling is evaluated 
strictly as a downstream post-translational Western blot endpoint in Aim 3.
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